July 24, 2020
Ok so Covid made Yannick et al head for the woods. But what about internet access?
It took some time but I figured it out. It is now fast and reliable. Low ping. Fast download. Fast upload. Amazing.
I suspect others in remote places could benefit from what I learnt. Two things were needed.
The MikroTik Chateau is incredible.
Put a SIM card inside, and its already much faster than:
But plug in a dual antenna and it’s crazy fast.
FYI, those 180 Mbps are despite having only 3 bars of reception.
It turns out that this router is Category 12 LTE, which means that it connects to the cell tower 3 times. So you get more combined bandwidth, and more resilience to interruptions - say if one of the cell tower’s were to become overloaded or fail.
MikroTik design and build these in Latvia. Most of their stuff is geared towards professionals. So the user interface offers immense flexibility - but is not easy to use. And it didn’t just “plug and play”.
FWIW the router is ~200 GBP in UK.
This had to be pointed at the nearest 4G phone tower - which I located using this handy map.
This made a huge improvement in cell reception.
(FWIW, I paid ~100 GBP for the antenna)
Every small thing you could want to imagine can be tuned on this router - and bazillions of things I am not even close to imagining (!).
However, it didn’t work right away. I had to specifically:
1. Set the APN
Following MikroTik’s help documents, I did this in the Terminal interface:
/interface lte apn add apn=internet.it use-network-apn=no /interface lte set lte1 apn-profiles=internet.it
2. In the Quick interface
3. In the WebFig interface
Now I just need to figure out how to correctly ground the antenna…
April 28, 2020
You know how a Y chromosome is usually smaller than an X chromosome, and it contains fewer genes?
We tested whether this pattern holds for the fire ant social chromosomes: is the b (which is similar to Y) smaller than the B (which is similar to X)?
Surprisingly, the opposite is true: the non-recombining b is almost twice as big as the B.
This is due to a process called “degenerative expansion”. We found the first evidence of it in an animal (there was a previous report in Papaya).
It turns out that immediately after recombination is suppressed, massive loss of genes is too costly.
Instead, degeneration of a non-recombining region occurs in 3 steps:
In most sex chromosome and other supergene systems, we have only seen the end of the 3rd phase. The systems studied are typically millions to hundreds of millions years old.
However, in the fire ant social supergene system we’ve been able to observe the first phase. This is probably for four reasons: the fire ant supergene is young, we used extra-long molecule sequencing, and ant males are haploid, making it easy to detect differences between haplotypes, and also creating strong purifying selection against gene loss.
For more details, check Eckart & Roddy’s paper: Degenerative expansion of a young supergene. Molecular Biology and Evolution 2019.
April 25, 2020
Long time no update (!)
Huge congratulations for the labs three new 2019 PhD graduates:
Similarly, big congrats to the four MSc students we hosted in 2019: Valentine Patterson and Richard Burns are now pursuing PhDs (respectively in Mainz and at Kings College). Iwo Pieniak and Catherine Okuboyejo are now data engineers.
All will be sorely missed.
Additional congrats to the lab’s former postdocs Joe Colgan and Eckart Stolle, who have or are both moved to new junior group leader positions in Germany.
Simultaneously we have welcomed a bunch of happy faces: Gabriel Hernandez-Gomez, Alicja Witwicka and Guy Mercer as new PhD students, and Anindita Brahma as a new Marie Sklodowska-Curie fellow. We look forward to working together!
As detailed on our phd and postdoc opportunities page, we are always eager to work with bright & motivated people, regardless what your background. Don’t hesitate to get in touch.
We will soon have an opening for a BBSRC-funded postdoctoral position looking at the molecular mechanisms underpinning the ability of pollinators to respond to environmental challenges. Stay tuned.
February 27, 2019
We have an exciting PhD position open through the London NERC DTP.
Apply by March 18th on the QMUL website.
The studentship is funded by the London NERC DTP will cover tuition fees and provide an annual tax-free maintenance allowance for 4 years at the Research Council rate (£17,009 in 2019/20). Candidates must meet RCUK eligibility criteria (I think this means ok for UK citizens and medium-term residents).
The project is highly interdisciplinary.
Great candidates fulfill at least 3 of the following 4 criteria:
We can adapt the project to the students’ interests and background.
If you have any questions regarding prerequisites, scope or nature of the project, please don’t hesitate to get in touch with me (Yannick).
We have two main lines of research, in collaboration with national and international colleagues and stakeholders.
Genetics of social behaviour. Social animals exhibit a broad range of behaviors, and some theoretical understanding exists of the tradeoffs between different forms of social organisation. However, we know little about the genes and processes underpinning social organisation or how it evolves. The diversity of social behaviors across the 20,000 species of ants represents a unique opportunity to empirically understand the mechanisms and tradeoffs involved in social change. We use highly molecular approaches, including genomics and bioinformatics but also potentially behavioural or field work to address major questions about social evolution. We aim to generate exciting new insights into genes and processes underpinning a major social transition, with implications on understanding evolution of complex phenotypes.
Molecular diagnostics for pollinator health. Effective pollination is crucial for the stability of the ecosystem, and for crop productivity. Governments had approved what they thought were “safe” levels of pesticides. But in fact, the pesticides are generic neurotoxins: they reduce the learning abilities, dexterity, foraging ability and ultimately survival of pollinators who consume nectar or pollen. As a result, several commonly used pesticides have now been banned. However, the problem may just have been shifted: we lack a good way of understanding whether authorised pesticides are better. Thus there is an urgent need for approaches that are more powerful/sensitive. The 50,000-fold drop in the cost of DNA sequence over the past 10 years has completely changed medical research and practice. Inspired by the changes, we are developing high-resolution molecular diagnostics approaches for pollinator health – these are poised to fundamentally change for the better how research on pesticides is performed and the mechanisms through which such crop chemicals are evaluated by regulatory agencies.
The student will receive extensive training in big data bioinformatics, phylogenomics, data visualisation, and experimental research approaches in evolution and genomics. Furthermore, they will receive hands-on training in interdisciplinary project management, communicating science in writing and verbally, including by presenting at workshops and conferences.
October 9, 2018
I (Yannick) spent the week of August 5th at the 18th Congress of the International Society for the Study of Social Insects in Guarujá, Brazil. This is a big quadrennial conference uniting researchers from around the world who study ants, bees, wasps, termites and a few other animals.
Part of my trip was funded by the Software Sustainability Institute which lobbies for and helps people do better research through improving software. Hence this blog post.
The study of social insects has traditionally used approaches including behavioral observation and taxonomic sampling, with genetic analyses becoming more common since the mid 2000s. A pleasant surprise at the conference was the recent increase in highly molecular, genome-wide approaches where whole or partial genomes or transcriptome sequences of many individuals are obtained in order to make specific comparisons within species, or sometimes also between species.
This disruptive shift is largely due to the 50,000-fold drop in DNA sequencing costs over the past 10 years. See Émeline’s recent review on the genes and processes underpinning evolution of social behavior in ants.
In light of this, as part of a workshop on genomics approaches organised with Tim Linksvayer and Alex Mikheyev, I gave an overview of some of the lessons we can transfer from the worlds of “other” data sciences to our expanding world of social insect genomics. This includes:
My slides are here:
It is worth highlighting three additional, important points raised during the congress that have more to do with interpretation, vocabulary and experimental design than anything technical:
A fun and highly stimulating conference.
October 1, 2018
How do you structure your files and folders for genomics analyses?
One challenge is that many analyses actually require multiple steps, thus having all steps in one place becomes a mess.
So we should structure our analyses across multiple folders. But how should we name them and keep track of their order?
Another (key) challenge in performing genomics analyses is that we often have to perform analyses multiple times.
The standard approach we use for all projects in the lab is derived from ideas initially proposed by William Noble in A Quick Guide to Organizing Computational Biology Projects. That initial model has been adjusted based on our experience of dozens of projects over the years, as well as discussions with Julien Roux, Anurag Priyam, and Roddy Pracana.
Best to just illustrate with an example of how this works at the simplest level.
2016-04-14-bombus_variant_calling ├── input │ ├── 2016-04-14-bombus_raw_28_samples │ │ ├── sample1.fq # could link to /data/SBCS-WurmLab/archive/db/genomic/reads/... │ │ ├── sample2.fq │ │ ├── sample3.fq │ │ ├── bombus_genome.fa -> ~/db/genomic/B_terrestris/Bter20110317-genome.fa │ │ └── WHATIDID.sh # list of ln -s, cp or wget/curl commands │ └── 2016-04-16-cleaned_reads │ ├── sample1.fq.gz -> ../../results/2016-04-14-read_cleaning/results/sample1.clean.fq.gz │ ├── sample2.fq.gz -> ../../results/2016-04-14-read_cleaning/results/sample2.clean.fq.gz │ ├── sample3.fq.gz -> ../../results/2016-04-14-read_cleaning/results/sample3.clean.fq.gz │ └── WHATIDID.sh # just the ln -s commands. ├── results │ ├── 2016-04-14-read_cleaning │ │ ├── input -> ../../input/2016-04-14-bombus_raw_28_samples │ │ ├── results # only few files here │ │ ├── sratoolkit -> ../../soft/sratoolkit-2.4.2/bin/ │ │ ├── tmp # use real scratch dir if more appropriate | | ├── ENVIRONMENT.sh # if any particular software, modules or containers need to be loaded │ │ └── WHATIDID.txt # or equivalent .sh or .Rmd (or knitr/jupyter) │ ├── 2016-04-16-mapping_to_reference │ │ ├── input -> ../../input/2016-04-16-cleaned_reads │ │ ├── results # only few files here │ │ ├── tmp # use real scratch dir if more appropriate | | ├── ENVIRONMENT.sh # if any particular software, modules or containers need to be loaded │ │ └── WHATIDID.txt # or equivalent .sh or .Rmd (or knitr/jupyter) │ └── WHATIDID.txt # for overall rationale └── soft ├── sratoolkit-2.4.2 # if installed locally ├── bwa -> /share/apps/sbcs/bwa/0.6.2/bin/bwa └── # links to other software if needed
resultsdir should contain a link named
inputto an input directory with a self explanatory name;
WHATIDID.txt(or an equivalent ruby/perl/jupyter/R/knitR/Sweave/Rmarkdown script) that contains all relevant commands. required to get from
July 5, 2018
Interested in supercharging the productivity of genome biologist researchers?
We have an exciting 4-year bioinformatics PhD position open through the London BBSRC LiDO Doctoral Training Programme.
Apply by 5pm July 20th here at LIDO to start in September.
A description of the project is below. It is highly interdisciplinary - no need to already be able to understand all the details today.
Great candidates fulfill 3 of the following four criteria: smart, hard working, understands genomes, and not scared of data analysis or coding.
If you have any questions regarding scope or nature of the project, or whether your skills are potentially sufficient, please don’t hesitate to get in touch with me (Yannick).
(Standard UKRI eligibility criteria apply (i.e. I think one must be UK resident). - the LiDO people can explain this better).
(apologies for the use of domain-specific jargon!)
The first generation of molecular-genetic research focused on traditional model organisms including mouse, yeast, zebrafish, Drosophila, and C. elegans. Genetic research increasingly uses diverse organisms that are much more relevant models for specific questions. For example, some such emerging organisms exhibit unique phenotypes including 100-fold intra-specific variation in lifespan, resistance to harsh environmental conditions, represent novel animal models for disease or development, provide crucial ecosystem services, or are key to food security because they are crops or may pollinate them.
A major challenge when working with such “emerging” model organisms is making sense of the “gene lists” that result from genome-wide analyses (e.g., of gene expression or genome-wide associations).
Here, we will develop a bioinformatics tool that takes a list of genes or genomic locations from a new species as input, and transparently produces relevant functional information describing this list of loci. When presented with data for which no direct information exists, the tool will in a first instance identify relationships of orthology to regions of other species. This will create a trail of links to databases in which functional information for orthologous regions does exist. These databases will be interrogated following hierarchical set of rules (initially defined based on human-curated examples). Using using cutting-edge “learning to rank” machine learning techniques the rulesets will be refined over time by tracking user behaviour (based on logs of which relationships/trails users retain) as well as explicitly allowing users to flag issues. The tool hereby makes it possible to extract significant value from largescale datasets that would otherwise require laborious case-by-case engineering efforts to connect. Summary data will be returned to the user using visualisations, statistics and tables in a manner that facilitates interpretation. Inferences and relationship calculations taking seconds will be available immediately; those taking minutes (e.g., distant orthology) will appear asynchronously as they complete; and those taking longer will result in email notification.
We will package our work in a manner that makes it accessible to biologists working with new or existing genomes. This builds on our extensive success with including with the SequenceServer and OMA software. Overall, our approach will substantially improve the ability of genome biologists to generate meaningful biological insight when working with new organisms.
This project is in collaboration with Christophe Dessimoz at UCL/Lausanne.
May 23, 2018
We have just posted a new manuscript to bioRxiv, where we describe the structural differences between the SB and Sb versions of the fire ant social chromosome pair.
We find that Sb is larger than SB and discuss how the suppression of recombination of Sb would lead to this type of ‘degenerative expansion’, as hypothesised for Y chromosomes and other non-recombining chromosomal regions. Read the manuscript, and tell us what you think!
May 18, 2018
This year the Wurm Lab were out in force at the annual Festival of Communities held in Stepney Green Park on Saturday 12 May. We brought some of our lab colonies of social invertebrates to demonstrate differences in social complexity including: Messor barbarus ants, Bombus terrestris bumblebees, and Stegodyphus dumicola social spiders. We had a fantastic time talking to the public about our research, taking groups around the park on minibeast safaris, and running a kids’ craft table where solitary bee hotels made from recycled drinks cartons were beautifully decorated. It was an immensely enjoyable day meeting people from the local community and some very enthusiastic and inspiring future scientists.
February 20, 2018
What is it? We called it the Index, it is our monthly reading review, tailored to our areas of interest and research.
Why do you do it? Because there are so many articles being published it can be quite difficult to keep up with reading new material. We want to increase our general knowledge of important questions, techniques and discoveries in our wider research areas while decreasing the likelihood of missing any relevant “key papers”. We are also keen to efficiently help each other out by sharing our readings. Finally, we are getting to read more broadly - including topics outside our comfort zone.
How does it work? Each month, each Wurmlab member gets a list of three journals to review from our own journal generator. The script is on Github here. Three articles that are relevant to the group or a particular lab member are picked form each journal’s table of contents. These articles are added to a document that anyone in the lab group can read at any time.
Great, where can I see an example? See the last instalment of the Index here.